Abstract

Background Thiripalai Mathirai (Tablet) (TPT) is a Siddha formulation, which consists of three fruits (Thiripala), namely Terminalia chebula (Retz.), Terminalia Belerica (Gaertn.)Roxb. & Emblica officinalis (Gaertn.) used for the treatment of Iron deficiency anaemia (Pitha pandu), Hyperlipidemia, Hepato Tonic, Antioxidant and Antidiabetic activity (Nadkarni, 1976). It was evaluated for Quantitative, Qualitative estimation of Phytochemical constituents and antioxidant activity. The antioxidant activity is confirmed by total phenol estimation by using FollinCio-caiteau method. The test drug was referred in the classical siddha text “Kadukkai Vallaraiyin Thani Maanbu” ( Hakkim.P.Mohammed Abdullah Sahib). Objective The objective of this study is to determine the antioxidant potential and phytochemicals in Thiripalai mathirai (TPT). Methods The qualitative phytochemical analysis were carried out for major compounds, such as alkaloid, phenols etc. The phytochemical analysis is detected by using Harborne and Onwukaemeand co-workers, 1999 methods. The Quantitative estimation were done through spectrophotometric methodology, detection of alkaloids and phenol estimation is using FolinCio-calteau methodolgy. Results The end of result concluded the presence of glycosides, alkaloids, flavonoids, phenols, terpenoids, steroids and tannins in the Thiripalai mathirai (TPT). The aqueous extract of alkaloid and total phenolic compound present in the sample was 3.2604mg and 0.52mg in 10mg respectively, which are expressed in caffeine units. Conclusion The presence of phytochemicals in quantitative estimation may scavenge the free radicals and it has synergetic activity as antioxidant which serves for the mankind. .