Abstract

Inflammatory response is a protective response to injury, irritant or infection and is required to remove the injurious stimuli as well as initiate the healing process. It is a complex process and it associated with increased vascular permeability, protein denaturation and membrane alteration. The common symptoms of arthritis is pain, which is due to the inflammation of joints line. Pirrandai chooranam is a polyherbal Siddha formulation, indicated in Siddha text Siddha vaithiya thirattu which contain 9 ingredients of plant origin. It was mainly indicated for the treatment of Vatham. The aim of the study is to evaluate the Anti- inflammatory activity of Anti-inflammatory activity by using Protein (Albumin) denaturation technique. The reaction mixture consisted of bovine serum albumin (5% aqueous solution) and test sample Pirrandai Chooranam (PC) at varying concentration ranges from 100 to 500 ?g/ml and standard Diclofenac sodium at the concentration of 100 ?g /ml. The results of the study showed that the test drug was effective in inhibiting heat induced albumin denaturation. The Concentration range of VKI at 100, 200, 300, 400 and 500 ?g/ml produce significant inhibition of protein denaturation in concentration dependent manner. Maximum percentage of protein inhibition about 74.25 ± 2.81 % was observed at 500 ?g/ml, when compared to Diclofenac sodium, a standard anti-inflammatory agent with the maximum inhibition about 96.57 ± 1.27 at the concentration of 100 ?g/ml. Hence this study result offers the Pirrandai Chooranam (PC) possess significant Anti- inflammatory activity in Protein (Albumin) denaturation technique.