A Quarterly Inter-disciplinary open access Journal

International Journal of Reverse Pharmacology and Health Research

ISSN 2589-3343

IJRPHR is an open access, peer-review journal, devoted to nurture the traditional medicines through reverse pharmacology concepts and implementing it to health care system through health research methods.

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Research article , : 2021 | Volume: 4 | Issue: 4 | Pages : 186-190

In-vitro protein denaturation inhibition assay of Sivappu thailam an anti-inflammatory Siddha therapeutic oil

Corresponding author: Archana K*, Mahadevan MV, Mahalakshmi V, Muthukumar NJ,

Name : Archana K
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The needs for herbal source of anti-inflammatory formulations are important to ease the pain of individuals. This could possibly due the known ill effect of currently available non-steroidal anti-inflammatory drugs (NSAIDS). This study is conducted to evaluate the In-vitro anti-inflammatory activity of Sivappu thailam (ST). The Objective of this study is to assess the anti-inflammatory of Sivappu thailam using Invitro Protein denaturation assay. The test drug Sivappu thailam at varying concentration ranges from 100 to 500μg/ml is incubated and heated with Egg albumin in controlled experimental conditions. The percentage inhibition of the protein denaturation is calculated using standard methods where Diclofenac sodium is used as reference standard. The result from the present study clearly states that the drug Sivappu thailam is effective in inhibiting heat induced protein denaturation. Turbidity developed was measured spectrophotometrically at 660nm and maximum percentage of inhibition of 50.91 ± 0.99 was observed at 500 μg/ml when concern with the Diclofenac sodium as 97.19 ± 3.80.


herbal, anti inflammatory,