Abstract

The test drug Vembu karpam composed of of chief ingredient Azadirachta indica which is referenced from Bogar Vaithiya Saram 700. It has wide clinical role and majorly used to alleviate vatha related disorders especially Uthara vatha suronitham.  The aim of the present study was to evaluate the in vivo acute oral toxicity Siddha herbal formulation Vembu karpam. Acute oral toxicity is carried out as per the OECD-423 guidelines after the animal ethical clearance from the Institutional Animal Ethics Committee. In the acute toxicity study female albino Mice administered single oral dose (0, 300, 2000 mg/kg) of Vembu karpam, and observed the physical symptoms and behavioral changes for the crucial phases of the study. In the acute toxicity study, no such mortality or behavioural changes were observed in the treated animals with a varied single dose of Vembu karpam upto 2000 mg/kg, which is considered as highly tolerable doses. These results exhibit the absence of acute oral toxicity after treatment of Vembu karpam in rats. However, further clinical studies humans are needed in order to have sufficient safety evidence for its use in humans.