BIOCHEMICAL ANALYSIS OF SIDDHA POLYHERBAL DRUG KUTHIKAAL VATHA KUDINEER

Sathiya  A 1, Tamilselvi  V 2, Muneeswaran  .A 3

 

1PG Scholar, Department of Varma  Maruthuvam

2 PG Scholar, Department of siddhar yoga Maruthuvam

3 Associate Professor , Department of Varma  Maruthuvam

 

Government  Siddha Medical College & Hospital, Palayamkottai, Tirunelveli

 

 

 

 

                                              ABSTRACT    

                                                                                                                                                                                                                                                                       

Siddha system is a traditional system of healing medicine in southern part of the India.Siddha system deals with many kind of treatment procedure like yogam ,varmam,massage techniques ,Nanju noi maruthuvam etc.According  to varmam it contains manipulation  techniques,internal and external medicines to treat the different type of disease with a wide range of drugs .Among vadha diseases Kuthikaal vatham  is most common type of  vadha disease .The aim of the study was qualitative analysis of  Kuthikaal vatha kudineer to treat the kuthikaal vatham .siddha varmam drug taken from a varmam  literature.The Biochemical analysis of the trial drug indicates the presence of  sulphate ,ferrous iron,unsaturation compound ,amino acid revealed  the effectiveness of therapeutic action in vatha disease especially in kuthikaal vatham.

 

Keywords: Kuthikaal vatham,Calcaneal spur, Biochemical Analysis, Siddha Medicine, Kuthikaal vatha kudineer

 

INDROUCTION:                                                               

 

Calcaneal spur is not a growth but a reaction to the local inflammation of the  planter  Fascia    and  ligaments with deposition of calcium at the site of the ligamentous attachments. The condition is caused by a spike of bone at the anterior edge of the calcaneum   .The symptoms are not due to the spike per  se but to the causative conditions.Pain is felt in the ball of the heel ,which is more during long periods of standing.   The severity  of the pain is not proportional  to the size of the spur

In Vatha noi nithanam 800 text, Kuthikaal vatha kudineer  is indicated for vatha diseases.

MATERIALS & METHODS :

KUTHIKAAL VATHA KUDINEERS

DRUGS

BOTANICAL NAME

PART USED

AMANAAKU

Ricinus communis

ROOT

AYIL

Holoptelea           integrifolia

BARK

VENKODIVELI

Plumbago Zeylanica

ROOT

SUKKU

Zingiber officinale

DRIED RHIZOME

THAZHUTHAZHAI

Cleodendrum phlomodis

ROOT

SITRAAMUTTI

Sida cordifolia

ROOT

NILAPANAI

Curculigo orchioides

TUBERS

KADUKKAI

Terminalia chebula

DRIED FRUITS

KANCHORI

Tragia involucrate

ROOT

CHEVIYAM              

                    

Piper nigrum

ROOT

KARUNKURINJI

      Ecbolium ligustrinum

ROOT

DEVADARU

    Cedrus deodara

       KATTAI

 

           


Source Of Drug Ingredients:

The required raw drugs for preparations of Kuthikaal vatha kudineer  are  purchased from a well reputed country shop. The purchased drugs are authenticated by The Faculty / Expert members of Medicinal Botanist  of GSMCH- Palayamkottai.

Methods Of Purification And Preprations:

All the ingredients has been  completely purified as per the siddha   literature   in the presence knowledge of Guide / Faculty members. The purified raw drugs are coarsely powdered and mixed in of each equal quantity.The decoction is made out from the above mixture as per the method available in siddha literatures

Biochemical analysis:                                                      

Screening  the  drug  Kuthikaal vatha kudineer  to  identify  the  Biochemical properties present in the ingredient.

Chemicals and drugs:

An  the  chemicals  used  in  this  study  were  of  analytical  grade  obtain  from Department of Biochemistry, Government Siddha Medical College & Hospital, Palayamkottai.

Methodology:

5  grams  of  the  drug  was  weighed  accurately  and  placed  in  a  250ml  clean beaker. Then 50ml of distilled water added to it and dissolved well. Then it was boiled well for about 10 minutes. It was cooled and filtered in a 100ml volumetric flask and then it is made upto 100ml with distilled water. This fluid was taken for analysis.

 

QUALITATIVE ANALYSIS

S.No

EXPERIMENT

OBSERVATION

INFERENCE

01

TEST FOR CALCIUM

2ml of the above prepared extract is taken in a clean test tube. To this add 2ml of 4% Ammonium oxalate solution

A  white precipitate is formed

 

Indicates the Presence of calcium

02

TEST FOR SULPHATE

2ml of the extract is added to 5% Barium chloride solution.

A white precipitate is formed

 

Indicates the presence of sulphate

03

TEST FOR CHLORIDE

The extract is treated with silver nitrate solution

A  white precipitate is formed

 

Indicates the Presence of chloride

04

TEST FOR CARBONATE

The substance is treated with concentrated Hcl.

No brisk effervessence is formed

Absence of carbonate

05

TEST FOR STARCH

The extract is added with weak iodine solution

Blue colour is formed

Indicates the presence of starch

06

TEST FOR FERRIC IRON

The extract is acidified with Glacial acetic acid and potassium ferro cyanide.

No blue colour is formed

Absence of ferric iron

07

TEST FOR FERROUS IRON

The extract is treated with concentrated Nitric acid and Ammonium thiocyanate solution

No Blood red colour is formed

Absence of ferrous iron

08

TEST FOR PHOSPHATE

The extract is treated with Ammonium Molybdate and concentrated nitric acid

No yellow precipitate is formed

Absence of phosphate

09

TEST FOR ALBUMIN

The extract is treated with Esbach’s reagent

No yellow precipitate is formed

Absence of albumin

10

TEST FOR TANNIC ACID

The extract is treated with ferric chloride.

blue black precipitate is formed

Indicate  the presence of Tannic acid

11

TEST FOR UNSATURATION

Potassium permanganate solution is added to the extract

It gets decolourised

Indicates the presence of unsaturated compound

12

TEST FOR THE REDUCING SUGAR

5ml of Benedict’s qualitative solution is taken in a test tube and allowed to boil for 2 minutes and add 8-10 drops of the extract and again boil it for 2 minutes.

Colour changes occur

Indicate  the presence of reducing sugar

 

13

TEST FOR AMINO ACID

One or two drops of the extract is placed on a filter paper and dried well. After drying, 1% Ninhydrin is sprayed over the same and dried it well.

Violet colour is formed

Indicates the presence of Amino acid

14

TEST FOR ZINC

The extract is treated with Potassium Ferro cyanide.

No white precipitate is formed

Absence of zinc

e


 

 

 

 

 

RESULTS AND DISCUSSION:

 

The Bio chemical analysis of the trial drug  Kuthikaal vatha kudineer   was tabulated above in table 2.

The trial drug , Kuthikaal vatha kudineer  contains,

                                                1.Calcium

 

                                                2.Starch            

 

                                               3.Sulphate

 

                                                4.Chloride

 

                                                   5.Unsaturated compounds

 

                                                   6.Aminoacid

 

                                                    7.Tannic acid

 

                                   8.Reducing sugar

                                                                                                               

mode of action of  the trial drug Kuthikaal vatha kudineer  which brings about the Bone Mineralisation osteoblastic  and  osteoclastic  activity  in  body.  May  be  due    the  presence  of    Sulphate, Amino acid, calcium in it

 

                 CONCLUSION:

 

Kuthikaal vatha kudineer  is  a  Siddha  Drug  taken  from  a  Siddha literature  used  in  the treatent of vatha diseases.The drug  is screened for its biochemical properties.Further comprehensive  pharmacological analysis are needed to evaluate its potency and the drug has its own potency to undergo further research.

 

 

 

 

 

 

                                    ACKNOWLEDGEMENT

 

 

The author  wish to acknowledge our  health thanks to Dr.A.Muneeswaran Head of the  department ,Dr.S.Sujatha,Lecturer,Department of Varma maruthuvam,and Department  of  Biochemistry,Government siddha medical college&Hospital ,Palayamkottai and thanks to my department faculties.

 

 

 

 

 

References:

 

1.Murugesa Mudaliar K.S.Textbook of  Materia Medica (Gunapadam)Mooligai ,Department of Indian Medicine and Homeopathy

2.Text book of Varma Maruthuvam.

3.Varma Marunthu  Seimuraigal

4..Davidson Textbook of Modern Medicine

5.Taxonomy of Angiosperm

6.Vatha noi nithanam

 

7.Anonymous sarakku suthi Muraigal ,First edition