BIOCHEMICAL ANALYSIS OF SIDDHA POLYHERBAL DRUG KUTHIKAAL VATHA KUDINEER
Sathiya A 1, Tamilselvi V 2, Muneeswaran .A 3
1PG Scholar, Department of Varma Maruthuvam
2 PG Scholar, Department of siddhar yoga Maruthuvam
3 Associate Professor , Department of Varma Maruthuvam
Government Siddha Medical College & Hospital, Palayamkottai, Tirunelveli
ABSTRACT
Siddha system is a traditional system of healing medicine in southern part of the India.Siddha system deals with many kind of treatment procedure like yogam ,varmam,massage techniques ,Nanju noi maruthuvam etc.According to varmam it contains manipulation techniques,internal and external medicines to treat the different type of disease with a wide range of drugs .Among vadha diseases Kuthikaal vatham is most common type of vadha disease .The aim of the study was qualitative analysis of Kuthikaal vatha kudineer to treat the kuthikaal vatham .siddha varmam drug taken from a varmam literature.The Biochemical analysis of the trial drug indicates the presence of sulphate ,ferrous iron,unsaturation compound ,amino acid revealed the effectiveness of therapeutic action in vatha disease especially in kuthikaal vatham.
Keywords: Kuthikaal vatham,Calcaneal spur, Biochemical Analysis, Siddha Medicine, Kuthikaal vatha kudineer
INDROUCTION:
Calcaneal spur is not a growth but a reaction to the local inflammation of the planter Fascia and ligaments with deposition of calcium at the site of the ligamentous attachments. The condition is caused by a spike of bone at the anterior edge of the calcaneum .The symptoms are not due to the spike per se but to the causative conditions.Pain is felt in the ball of the heel ,which is more during long periods of standing. The severity of the pain is not proportional to the size of the spur
In Vatha noi nithanam 800 text, Kuthikaal vatha kudineer is indicated for vatha diseases.
MATERIALS & METHODS :
KUTHIKAAL VATHA KUDINEERS
DRUGS |
BOTANICAL NAME |
PART USED |
AMANAAKU |
Ricinus communis |
ROOT |
AYIL |
Holoptelea integrifolia |
BARK |
VENKODIVELI |
Plumbago Zeylanica |
ROOT |
SUKKU |
Zingiber officinale |
DRIED RHIZOME |
THAZHUTHAZHAI |
Cleodendrum phlomodis |
ROOT |
SITRAAMUTTI |
Sida cordifolia |
ROOT |
NILAPANAI |
Curculigo orchioides |
TUBERS |
KADUKKAI |
Terminalia chebula |
DRIED FRUITS |
KANCHORI |
Tragia involucrate |
ROOT |
CHEVIYAM
|
Piper nigrum |
ROOT |
KARUNKURINJI |
Ecbolium ligustrinum |
ROOT |
DEVADARU |
Cedrus deodara |
KATTAI |
Source Of Drug Ingredients:
The required raw drugs for preparations of Kuthikaal vatha kudineer are purchased from a well reputed country shop. The purchased drugs are authenticated by The Faculty / Expert members of Medicinal Botanist of GSMCH- Palayamkottai.
Methods Of Purification And Preprations:
All the ingredients has been completely purified as per the siddha literature in the presence knowledge of Guide / Faculty members. The purified raw drugs are coarsely powdered and mixed in of each equal quantity.The decoction is made out from the above mixture as per the method available in siddha literatures
Biochemical analysis:
Screening the drug Kuthikaal vatha kudineer to identify the Biochemical properties present in the ingredient.
Chemicals and drugs:
An the chemicals used in this study were of analytical grade obtain from Department of Biochemistry, Government Siddha Medical College & Hospital, Palayamkottai.
Methodology:
5 grams of the drug was weighed accurately and placed in a 250ml clean beaker. Then 50ml of distilled water added to it and dissolved well. Then it was boiled well for about 10 minutes. It was cooled and filtered in a 100ml volumetric flask and then it is made upto 100ml with distilled water. This fluid was taken for analysis.
QUALITATIVE ANALYSIS
S.No |
EXPERIMENT |
OBSERVATION |
INFERENCE |
01 |
TEST FOR CALCIUM 2ml of the above prepared extract is taken in a clean test tube. To this add 2ml of 4% Ammonium oxalate solution |
A white precipitate is formed
|
Indicates the Presence of calcium |
02 |
TEST FOR SULPHATE 2ml of the extract is added to 5% Barium chloride solution. |
A white precipitate is formed
|
Indicates the presence of sulphate |
03 |
TEST FOR CHLORIDE The extract is treated with silver nitrate solution |
A white precipitate is formed
|
Indicates the Presence of chloride |
04 |
TEST FOR CARBONATE The substance is treated with concentrated Hcl. |
No brisk effervessence is formed |
Absence of carbonate |
05 |
TEST FOR STARCH The extract is added with weak iodine solution |
Blue colour is formed |
Indicates the presence of starch |
06 |
TEST FOR FERRIC IRON The extract is acidified with Glacial acetic acid and potassium ferro cyanide. |
No blue colour is formed |
Absence of ferric iron |
07 |
TEST FOR FERROUS IRON The extract is treated with concentrated Nitric acid and Ammonium thiocyanate solution |
No Blood red colour is formed |
Absence of ferrous iron |
08 |
TEST FOR PHOSPHATE The extract is treated with Ammonium Molybdate and concentrated nitric acid |
No yellow precipitate is formed |
Absence of phosphate |
09 |
TEST FOR ALBUMIN The extract is treated with Esbach’s reagent |
No yellow precipitate is formed |
Absence of albumin |
10 |
TEST FOR TANNIC ACID The extract is treated with ferric chloride. |
blue black precipitate is formed |
Indicate the presence of Tannic acid |
11 |
TEST FOR UNSATURATION Potassium permanganate solution is added to the extract |
It gets decolourised |
Indicates the presence of unsaturated compound |
12 |
TEST FOR THE REDUCING SUGAR 5ml of Benedict’s qualitative solution is taken in a test tube and allowed to boil for 2 minutes and add 8-10 drops of the extract and again boil it for 2 minutes. |
Colour changes occur |
Indicate the presence of reducing sugar |
13 |
TEST FOR AMINO ACID One or two drops of the extract is placed on a filter paper and dried well. After drying, 1% Ninhydrin is sprayed over the same and dried it well. |
Violet colour is formed |
Indicates the presence of Amino acid |
14 |
TEST FOR ZINC The extract is treated with Potassium Ferro cyanide. |
No white precipitate is formed |
Absence of zinc |
e
RESULTS AND DISCUSSION:
The Bio chemical analysis of the trial drug Kuthikaal vatha kudineer was tabulated above in table 2.
The trial drug , Kuthikaal vatha kudineer contains,
1.Calcium
2.Starch
3.Sulphate
4.Chloride
5.Unsaturated compounds
6.Aminoacid
7.Tannic acid
8.Reducing sugar
mode of action of the trial drug Kuthikaal vatha kudineer which brings about the Bone Mineralisation osteoblastic and osteoclastic activity in body. May be due the presence of Sulphate, Amino acid, calcium in it
CONCLUSION:
Kuthikaal vatha kudineer is a Siddha Drug taken from a Siddha literature used in the treatent of vatha diseases.The drug is screened for its biochemical properties.Further comprehensive pharmacological analysis are needed to evaluate its potency and the drug has its own potency to undergo further research.
ACKNOWLEDGEMENT
The author wish to acknowledge our health thanks to Dr.A.Muneeswaran Head of the department ,Dr.S.Sujatha,Lecturer,Department of Varma maruthuvam,and Department of Biochemistry,Government siddha medical college&Hospital ,Palayamkottai and thanks to my department faculties.
References:
1.Murugesa Mudaliar K.S.Textbook of Materia Medica (Gunapadam)Mooligai ,Department of Indian Medicine and Homeopathy
2.Text book of Varma Maruthuvam.
3.Varma Marunthu Seimuraigal
4..Davidson Textbook of Modern Medicine
5.Taxonomy of Angiosperm
6.Vatha noi nithanam
7.Anonymous sarakku suthi Muraigal ,First edition