BIOCHEMICAL ANALYSIS OF SIDDHA MONOHERBAL DRUG AMMAIYAAR KOONTHAL CHOORANAM

Sagaya Shemina Joice G 1, Tamila R 2, Ahamed Mohideen M 3

1 PG Scholar, Department of Pura Maruthuvam

² PG Scholar, Department of Pura Maruthuvam

3 Associate Professor, Department of Pura Maruthuvam

Government Siddha Medical College & Hospital , Palayamkottai, Tirunelveli.

ABSTRACT

Siddha system is a traditional system of healing medicine in southern part of the India. Osteoarthritis is a common degenerative joint disease and major cause of pain and disability in adult individuals is seen now a day. In the present case study, a diagnosed case of osteoarthritis has been included for its siddha management

Keywords: Osteoarthritis, Biochemical Analysis, Siddha Medicine, Ammaiyaar Koonthal chooranam

INTRODUCTION:

Osteoarthritis is a chronic degenerative disease affecting mainly the articular cartilage of the big weight bearing joints of the body. It is the most common form of arthritis characterized by subchondral sclerosis and changes in the soft tissues including the synovial membrane joint capsule, ligaments and muscle synovial inflammation. knee pain is the activities and drives them who consult a doctor.

Osteoarthritis is a major to functional impairment and reduced independence in older adults so with this background, the disease osteoarthritis in knee taken for the study.

In Gunapadam Mooligai Vaguppu part 1 text, Ammaiyaar Koonthal Chooranam is indicated for vatha diseases.

MATERIALS & METHODS: PERARATHAI CHOORANAM

Sl. No	DRUGS	BOTANICAL NAME	PART USED	Quantity
1	AMMAIYAAR KOONTHAL	Cuscuta reflexa	whole plant	1 PART

Source of Drug Ingredients:

The drug are purified as per the evidence mentioned in the sarakku suthi muraigal and anuboga vaithya bramma ragasiyam. The drugs are authenticated by The Faculty / Expert members of Medicinal Botany and Gunapadam department at GSMCH- Palayamkottai.

Methods of Purification and Preparations:

All the ingredients have been completely purified as per the siddha literature in the presence knowledge of Guide / Faculty members. Then the trail drug is prepared from the ingredients.

Biochemical analysis:

Screening the drug Ammaiyaar koonthal Chooranam to identify the Biochemical properties present in the ingredient.

Chemicals and drugs:

The chemicals used in this study were of analytical grade obtain from Department of Biochemistry, Government Siddha Medical College & Hospital, Palayamkottai.

Methodology:

5 grams of the drug was weighed accurately and placed in a 250ml clean beaker. Then 50ml of distilled water added to it and dissolved well. Then it was boiled well for about 10 minutes. It was cooled and filtered in a 100ml volumetric flask and then it is made up to 100ml with distilled water. This fluid was taken for analysis.

QUALITATIVE ANALYSIS

S.No	EXPERIMENT	OBSERVATION	INFERENCE
01	TEST FOR CALCIUM 2ml of the above prepared extract is taken in a clean test tube. To this add 2ml of 4% Ammonium oxalate solution	A white precipitate is formed	Indicates The presence of calcium
02	TEST FOR SULPHATE 2ml of the extract is added to 5% Barium chloride solution.	A white precipitate is formed	Indicates the presence of sulphate
03	TEST FOR CHLORIDE The extract is treated with silver nitrate solution	A white precipitate is formed	Indicates the Presence of chloride
04	TEST FOR CARBONATE The substance is treated with concentrated HCL.	No brisk effervescence is formed	Absence of carbonate
05	TEST FOR STARCH The extract is added with weak iodine solution	Blue color is formed	Indicates the presence of starch
06	TEST FOR FERRIC IRON The extract is acidified with Glacial acetic acid and potassium ferro cyanide.	No blue color is formed	Absence of ferric iron
07	TEST FOR FERROUS IRON The extract is treated with concentrated Nitric acid and Ammonium thiocyanate solution	No Blood red color is formed	Absence of ferrous iron
08	TEST FOR PHOSPHATE The extract is treated with Ammonium Molybdate and concentrated nitric acid	No yellow precipitate is formed	Absence of phosphate
09	TEST FOR ALBUMIN The extract is treated with Eshbach’s reagent	No yellow precipitate is formed	Absence of albumin
10	TEST FOR TANNIC ACID The extract is treated with ferric chloride.	No blue-black precipitate is formed	Absence of Tannic acid
11	TEST FOR UNSATURATION Potassium permanganate solution is added to the extract	It gets decolorized	Indicates the presence of unsaturated compound
12	TEST FOR THE REDUCING SUGAR 5ml of Benedict’s qualitative solution is taken in a test tube and allowed to boil for 2 minutes and add 8-10 drops of the extract and again boil it for 2 minutes.	No Color change occurs	Absence of reducing sugar

13	TEST FOR AMINO ACID One or two drops of the extract is placed on a filter paper and dried well. After drying, 1% Ninhydrin is sprayed over the same and dried it well.	Violet color is formed	Indicates the presence of Amino acid
14	TEST FOR ZINC The extract is treated with Potassium Ferro cyanide.	No white precipitate is formed	Absence of zinc

RESULTS AND DISCUSSION:

The Bio chemical analysis of the trial drug Ammaiyaar Koonthal chooranam was tabulated above in table.

The trial drug, Ammaiyaar Koonthal chooranam contains,

1. Calcium

2. Sulphate

3. Chloride

4. Starch

5. Unsaturated Compounds

6. Amino Aci

mode of action of the trial drug Ammaiyaar Koonthal chooranam which brings about the Bone Mineralisation osteoblastic and osteoclastic activity in body. May be due to the presence of Sulphate, Amino acid, calcium in it.

Conclusin:

Ammaiyaar Koonthal chooranam is a Siddha Drug taken from a Siddha literature used in the treatment of vatha diseases. The drug is screened for its bio chemical properties. Further, comprehensive pharmacological analysis is needed to evaluate its potency and the drug has its own potency to undergo further research