Biochemical Analysis Of Siddha Monoherbal Drug Perarathai Chooranam

Tamila R 1, Sagaya Shemina Joice G 2, Ahamed Mohideen M 3

1 pg scholar, Department of pura maruthuvam

² pg scholar, Department of pura maruthuvam

3 Associate professor , Department of pura maruthuvam

Government siddha medical college & hospital , palayamkottai, tirunelveli

Abstract

Siddha system is one of the oldest system of medicine which taught by super natural people[siddhars] to embhasis health as the perfect state of physical ,mental ,social,moral and spiritual well being of humen beings.compelling evidence as shown that the incidence of cervical spondylosis increases with age.it is a essential to note the relationship between the age and the incidence of cervical spondylosis through more and more clinical data. In the present case study, a diagnosed case of cervical spondylosis has been included for its siddha management.

Keywords: cervical spondylosis, biochemical analysis, siddha medicine, perarathai chooranam

Introduction:

Cervical spondylosis is a chronic degenerative process of cervical spine that affects the vertebral bodies and intervertebral disc of the cervical spine and may progress into disc herniation,bone spur formation,compression of spinal cord[myelopathy],compression of nerve root[radiculopathy].Spondylotic changes leads to stenosis of spinal canal ,lateral recess and foraminae which resulting in cervical spondylosis myelopathy.Radiculopathy is a result of intervertebral foraminae narrowing.In the case of Myeloradiculopathy patients have problem with bladder function.

In Gunapadam Mooligai Vaguppu Part 1 text, Perarathai Chooranam is indicated for Vatha Diseases.

Materials & methods : perarathai chooranam

Drugs	Botanical name	Part used	Quantity
Perarathai	Alpinia galanga	Root	1 part

Source of drug ingredients:

The required raw drugs for preparations of perarathai chooranam are purchased from a well reputed country shop. The purchased drugs are authenticated by the faculty / expert members of medicinal botany and gunapadam department at gsmch- palayamkottai.

Methods of purification and preprations:

All the ingredients has been completely purified as per the siddha literature in the presence knowledge of guide / faculty members. Then the trail drug is prepared from the ingredients.

Biochemical analysis:

Screening the drug perarathai chooranam to identify the biochemical properties present in the ingredient.

Chemicals and drugs:

An the chemicals used in this study were of analytical grade obtain from department of biochemistry, government siddha medical college & hospital, palayamkottai.

Methodology:

5 grams of the drug was weighed accurately and placed in a 250ml clean beaker. Then 50ml of distilled water added to it and dissolved well. Then it was boiled well for about 10 minutes. It was cooled and filtered in a 100ml volumetric flask and then it is made upto 100ml with distilled water. This fluid was taken for analysis.

Qualitative analysis

S.no	Experiment	Observation	Inference
05	Test for starch The extract is added with weak iodine solution	Blue colour is formed	Indicates the presence of starch
06	Test for ferric iron The extract is acidified with glacial acetic acid and potassium ferro cyanide.	No blue colour is formed	Absence of ferric iron
07	Test for ferrous iron The extract is treated with concentrated nitric acid and ammonium thiocyanate solution	Blood red colour is formed	Indicates the presence of ferrous iron
08	Test for phosphate The extract is treated with ammonium molybdate and concentrated nitric acid	No yellow precipitate is formed	Absence of phosphate
09	Test for albumin The extract is treated with esbach’s reagent	No yellow precipitate is formed	Absence of albumin
10	Test for tannic acid The extract is treated with ferric chloride.	No blue black precipitate is formed	Absence of tannic acid
11	Test for unsaturation Potassium permanganate solution is added to the extract	It gets decolourised	Indicates the presence of unsaturated compound
12	Test for the reducing sugar 5ml of benedict’s qualitative solution is taken in a test tube and allowed to boil for 2 minutes and add 8-10 drops of the extract and again boil it for 2 minutes.	Colour change occurs	Indicate the presence of reducing sugar
13	Test for amino acid One or two drops of the extract is placed on a filter paper and dried well. After drying, 1% ninhydrin is sprayed over the same and dried it well.	Violet colour is formed	Indicates the presence of amino acid
14	Test for zinc The extract is treated with potassium ferro cyanide.	No white precipitate is formed	Absence of zinc

Results and discussion:

The bio chemical analysis of the trial drug perarathai chooranam was tabulated above in table .

The trial drug , perarathai chooranam contains,

1.sulphate

2.chloride

3.starch

4.ferrous iron

5.unsaturated compounds

6.reducing sugar

7.amino acid

Mode of action of the trial drug perarathai chooranam which brings about the bone mineralisation osteoblastic and osteoclastic activity in body. May be due to the presence of sulphate, amino acid, calcium in it.

Conclusion:

Perarathai chooranam is a siddha drug taken from a siddha literature used in the treatment of vatha diseases. The drug is screened for its bio chemical properties. Further, comprehensive pharmacological analysis are needed to evaluate its potency and the drug has its own potency to undergo further research.