Preliminary Qualitative chemical Analysis of Poly herbal formulation “Cuttira Apaiyati Lekiyam”.
Devishree L1*, Esakki muthu K1,Soundararajan D K 2
1* PG Scholars, Department of Kuzhandhai Maruthuvam, Government Siddha Medical College and Hospital, Palayamkottai.
2 Professor and Head of the Department ,Department of Kuzhandhai Maruthuvam, Government Siddha Medical College And Hospital, Palayamkottai.
ABSTRACT
Siddha system of medicine is the oldest system of medicine in india.18 Siddhars were contributed for the development of this system. Siddha medicine is capable of treating different types of paediatric disease. pandu noi is one among them. The disease pandu noi is the nearest correlation of anemia.
Anemia is a condition in which the number of red blood cells or haemoglobin in the blood is low. The most common cause of anemia is thought to be iron deficiency.Children are particularly vulnerable to iron deficiency because of their increased iron requirements in the period of rapid growth. Iron deficiency anemia in children has been linked to increased childhood morbidity and impaired cognitive development so it is important to treat anemia in children as soon as possible.
There are huge medicines for the treatment of pandu noi in Siddha and this article aims about phytochemical analysis of poly herbal formulation “Cuttira Apaiyati Lekiyam”.the analysis of the drug will helpful in further studies.
Keywords:
Pandu noi, anemia, Cuttira apayati lekiyam, Children, Biochemical analysis
INTRODUCTION
Anemia is a decrease in the total amount of red blood cells or haemoglobin. Haemoglobin is a type of protein that allows red blood cells to carry oxygen to other cells in the body. Iron deficiency anemia is a major health problem in children. Over the last 50years the prevalence of iron deficiency anemia in children was ranged between 68% to 97%.who has estimated that globally 293million of children were affected by anemia.
According to 4th National Family Health Survey (2015-2016)data in tamilnadu the prevalence of anemia among children in the age group of 6-59months is about 50.4%
Risk factors for anemia includes premature or low birth weight , living in poverty, early use of cow’s milk ,low iron diet, surgery or accidental blood loss, long term illness such as infections or kidney or liver disease
Siddha Aspect
Pandu noi is a disease of Rattha thathu, characterised by pallor of skin, nails, conjunctiva and tongue. Pandu literally means pallor and it is of Sanskrit origin meaning pale skinned man the first references being found in great epic Mahabharata. When vyasa approached ambalika she got frightened due to his scary appearance she had become pale hence her son was born pale thus he was named as pandu.
Etiology :
Excessive intake of salt sour foods,muds,ashes,betel leaves,betel-nuts
Hemorrhagic condition like Mennorhagia(perumpadu), Hypertension(kurudhiazhal) ,Haemorrhoids(moolam), Hematemesis(kuruthivaandhi)
Fever, Diarrhoea, Dysentery
Excessive intake of toxic drugs
Worm infestation
Hepatic disorders
Premonitory symptoms:
Dietary changes, derangements of pitha affect the colour and consistency of the blood which will prevent the proper supply of nutrients to the body
Dyspnoea while walking, weakness of lower limbs
Anorexia, Nausea, giddiness, frequent fainting ,weight loss, palpitation
Classification of pandu noi
Vatha pandu
Pitha pandu
Iyya pandu
Mukkutra pandu
Nanju pandu
Mannun pandu
MATERIALS AND METHODS
Cuttira Apaiyati Lekiyam
Noiyakandam kathiriver nutrumuppa thambalangal ayyakaduk kainuru palangal-paiyaa narukiyae enmarakkal narsalam vittae yerithuneer naalondraai yendhu.
Yendhum kudinneeri l yermoondra manduvellam Botthanee rikaraithup bothavidu-sendhakarka melamila vanga menthiri kadugu kola adimadhuram kuru
kurusiru nagapoo kollaveti vervagaike yerupala mondraekal yitthanaiyum- chooranamai paagathanil thoovip bathathiraki matranal vaagupera naalithen vaar.
Vaarumindha yoogapper vaiyakathil choothira seru mpaiyati lekiyamam –neerum porundhukudi neervendha bongkadukkai ondrum thirundhiyidu lekiyamum thin.
Thinathan soolaiyudan sersuvasa kasam Pannu marusi palaveekam –thunni Varundhum soolaipandu vanmoolam kunmam Pirindhasayam vandhiyinum pesu.
Pesupitham vaivuvikkal pedhasuram dheerndhuvidum dhesu tharumudambil dheebanamam – pesu malasuthi thadhuviruthi mayavudal pooti nalamundai immarundhai nadu
-Ref( Agasthiyar vaithiya sindhamani 4000)
Name |
Botanical name |
Family |
Parts used |
Kantankathiri ver |
Solanum surrattense |
Solanaceae |
Root |
kadukkay |
Terminalia chebula |
Combretaceae |
Fruit |
Neer |
Water |
||
Vellam |
Jaggery |
||
Elam |
Elettaria cardamomum |
Zingiberaceae |
Dried fruit |
Ilavankam |
Syzygium aromaticum |
Myrtaceae |
Flower bud |
Cukku |
Zingiber officinale |
Zingiberaceae |
Rhizome |
Milaku |
Piper nigrum |
Piperaceae |
Dried fruit |
Tippili |
Piper longum |
Piperaceae |
Fruit |
Atimaturam |
Glycyrrhiza glabra |
Fabaceae |
Root |
Cirunaka poo |
Cinnamomum wightii |
Lauraceae |
Flower bud |
Vettiver |
Vettiveria zizaniodus |
Poaceae |
Root |
Then |
Honey |
Table:1 Ingredients of Cuttira Apaiyati Lekiyam
Collection, identification and authentication of the drug: Kantankathiri ver were collected from Devadanapatti, Theni district. Remaining raw drugs were purchased from a well reputed country shop in theni.they were identified and authenticated by Botanist of Government Siddha Medical College and Hospital, Palayamkottai.
Purification of the drug: All the ingredients of this herbal formulation were purified according to the proper produce methods described in Siddha literature.
Preparation of the drug:
Make decoction of 1 and 2
After that add jaggery to the decoction and it is heated till it reaches pagu patham
Then add remaining powdered ingredients 5 to 11mix well till it reaches lekiyam consistency and allow it to cool.
Next day add honey on it mix well and store it in airtight container.
Administration of drug :
Form of the medicine : Lekiyam Dosage : 2.5gms twice a day depends on age Adjuvant : kadukkay kudineer Indication : Pandu, kamalai, soolai, suvaiyinmai, moolam,vaandhi, pithavaayu.
Chemical and drugs:
The chemical used in this study were of analytical grade obtained from Department of Biochemistry, Government Siddha Medical College And Hospital, Palayamkottai.
Biochemical analysis: Bio chemical screening of the extract gives idea regarding the chemical constituents present in trial drug.
Preparation of extract :
5gms of the drug was weighted accurately and placed in a 250 ml clean beaker then 50 ml of distilled water is added and dissolved well. Then it is boiled well for about 10minutes.it is cooled and filtered in a 100ml volumetric flask and then it is making up to 100ml with distilled water. This preparation was used for the biochemical analysis.
Table 2. Qualitative biochemical analysis of Cuttira Apaiyati Lekiyam
S.no |
EXPERIMENT |
OBSERVATION |
INFERENCE |
1. |
Test for Reducing sugars : Benedict’s test: the filtrate 1ml was treated with Benedict’s reagent and heated gently |
orange red precipitate is formed |
Indicates presence of reducing sugar. |
2. |
Test for Amino acids : One or two drops of the extract is placed on a filter paper and dried well. After drying, 1% Ninhydrin is sprayed over the filter paper and again dried it well. |
Violet colour is formed |
Indicates presence of amino acid.
|
3. |
Test for Tannic acid : The extract is treated with ferric chloride |
Blue black precipitate is formed |
Indicates presence of tannic acid |
4. |
Test for Starch : The extract is added with weak iodine solution |
blue colour is formed |
Indicates presence of starch.
|
5. |
Test for unsaturated compound: Baeyer’s test : Potassium permanganate solution is added to the extract. |
It gets decolorized |
Indicates the Presence of Unsaturated com- pound |
6. |
Test for calcium: 2ml of the above prepared extract is taken in a clean test tube. To this add 2ml of 4% Ammonium oxalate solution |
A white precipitate is formed |
Indicates the presence of Calcium |
7. |
Test for sulphate: 2ml of the extract is added to 5% Barium chloride |
A white precipitate is formed |
Indicates the presence of Sulphate |
8. |
Test for chloride: The extract is treated with silver nitrate solution |
No white precipitate is formed |
Absence of Chloride |
9. |
Test for carbonate: The substance is treated with concentrated Hcl. |
No brisk effervescence is formed |
Absence of Carbonate |
10. |
Test for ferrous iron: The extract is treated with concentrated Nitric acid and Ammonium thiocyanate solution. |
Blood red colour is formed |
Indicates the presence of Ferrous Iron |
11. |
Test for phosphate: The extract is treated with Ammonium Molybdate and concentrated nitric acid. |
No yellow precipitate is formed |
Absence of Phosphate |
12. |
Test for zinc: The extract is treated with Potassium Ferro cyanide. |
No white precipitate is formed |
Absence of Zinc |
13. |
Test for albumin: The extract is treated with Esbach’s reagent. |
No yellow precipitate is formed |
Absence of Albumin |
14. |
Test for ferric iron: The extract is acidified with glacial acetic acid and potassium ferrocyanide |
No blue colour is formed |
Absence of ferric iron |
Results and Discussion
The biochemical analysis of trial drug was tabulated above in table 2 the trial drug contains:
Calcium
Sulphate
Ferrous iron
Starch
Tannic acid
Unsaturated compound
Amino acid
Reducing sugar
these elements plays therapeutic role in the body and may helps in production of haemoglobin
Conclusion
The overall results of the study suggest that this drug contains few biochemical in them. the drug is easily available to prepare and cost effective and also safer for treatment. further pharmacological studies help in exploring this herbal Siddha formulation.
References
Prema S, Agasthiyar mani 4000 ennum vaithiya sindhamani venba 4000, part1,Thamarainoolakam,Chennai. March-1994,259-261.
Sambasivampillai T.V, Siddha Medical Dictionary Vol V, Department of Indian Medicine and Homeopathy, Chennai, Edition II,2006.
Dr. U.Sathyanarayana, Biochemistry 3rd Edition 2009, Arunabha Sen ,Books and Allied private limited.
Parthasarathy. IAP Text book of paediatrics, Jaypee brothers medical publishers, National Publication house, Gwalior 5th Edition, 649-655.
Kuppusamy Mudaliyar. Ka. Na.(2012).Siddha Medicine –Pothu, Department of Indian System of Medicine and Homeopathy, Govt.of Tamilnadu.chennai.
Murugesa Mudaliyar.,C (2008).Siddha Materia Medica part I Edition 2.Dept of Indian system of Medicine and Homeopathy, Govt.of Tamilnadu.chennai.
Dr.S. Somasundharam, Taxonomy of angiosperms,part2,Elangovan publication,march2011
Dr. S. Somasundharam, Medicinal Botany,Vol1, Elangovan publication, 5 thEdition,2009
An evaluation of physicochemical analysis and phyto chemical analysis of Siddha poly herbal formulation “Siringiparethi chooranam”
Int.J.Adv.Res.Biol.Sci. 6(2):24-37,2019.
N.V.Bhagavan,Chung- Eun Ha, Essential of Medical Biochemistry, ElsevierInc Metabolism of iron and heme(Chapter27).2nd Edition.,2015.