FTIR Characterization of Siddha Medicine Chitrarathai Chooranam

A.Gayathri1, A.Kingsly2

1.P.G Scholar,Department of Gunapadam, Govt Siddha Medical College, Palayamkottai.

2.Reader and HOD, Department of Gunapadam, Govt Siddha Medical College, Palayamkottai.

Abstract

Background: The Chitrarathaichooranam is a herbal medicine used for treating cough, eczema, oedema, dental diseases and used as expectorant also [1].

Objectives: To characterize the herbal drug “Chitrarathaichooranam

Materials and Methods : The ingredient used in this preparation is Chitrarathai root. The drugs was prepared as per siddha literatureGunapadamMooligaivakuppu. The drugs was analyzed by using FTIR Spectrum.

Result: FTIR characterization shows the presence of some functional group such asAlcohol, Alkane, Silicon, Cyclopentanone, Conjucated alkane, Alkene, Amine, Tertiary alcohol, Fluoro compound, Halo compound, 1,2-disubstitutes where identified in Siddha herbal formulation “ Chitrarathai chooranam”. If further research will be followed by the results based on this research work, it helps to utilize the medicinal effect of the siddha drug clinically in a safe manner.

Conclusion: The instrumental analysis FTIR study for Chitrarathaichooranam shows the presence of functional groups through the stretch and bends which responsible for its functional activity. These findings will help for further preclinical and clinical studies in Chitrarathaichooranam.

Keywords : FTIR analysis, Siddha formulation, Herbal medicine, Functional group

INTRODUCTION

Herbals are widely used in traditional siddha medicine. For getting more scientific knowledge for traditional medicines need much more investigations. Various medicinal compounds were identified in herbals. Those are always considered as safe with great medicinal value. The pharmaceutical principles of siddha medicines is a specialized one.

Chitrarathaichooranam was indicated as good siddha preparation for its wide medicinal uses. In classical days it is widely used as a drug to treat cough, eczema, edema, dental diseases and used as expectorant also[1].

The FTIR characterization were carried out to standardize this formulation. This study will helpful for structural and functional determination of this drug in various ways.

MATERIALS AND METHODS

Collection of raw materials

Chitrarathai root was collected from surrounding places near Nagercoil.

Chitrarathaichooranam is a siddha herbal formulation has the ingredients of

S.No.

Siddha name

Scientific name

Quantity

1.

Chitrarathai

Alpiniaofficinarum[2], [3]

S.Q

Process of preparation

Purified the Chitrarathai root by removing the outer layer and dried it. Then it is placed in iron mortor and powdered finely[1],[4].Then it was sieved through the cotton white cloth. Finallychooranam was obtained and stored in an airtight container.

Dose

1 – 2 gm

Adjuvant

Honey – Twice or Thrice a day after food[1].

Indication

Cough, eczema, edema, dental diseases and used as expectorant also [1].

Details regarding the analysis

FTIR spectra were recorded at kalasalingam academy of research and education (International research center) Srivilliputhur.

FTIR Spectrum analysis

Fourier transform infrared spectroscopy it is an important and more advanced technique. It is used to identify the functional group to determine the quality and consistency of the sample material and can determine the amount of compound present in the sample.

In FTIR- infrared is passed from a source through a sample. This infrared is absorbed by the sample according to the chemical properties and some are transmitted. The spectrum that appears denotes the molecular absorption and transmission. It forms the molecular finger print of the sample. It is recorded as wavelength and the peaks seen in the spectrum indicate the amount of material present.

RESULTS

Fig.1. Image of the FTIR spectrum.

Table.1: FTIR Data interpretation of SAMPLE (AOP)

Wave number

Vibrational modes of SAMPLE (AOP) In IR region

Functional groups

3778.55

O-H Stretching

Alcohol

3535.52

O-H Stretching

Alcohol

3010.88

C-H Stretching

Alkane

2926.01

C-H Stretching

Alkane

2854.65

C-H Stretching

Alkane

2357.01

Si-HStreching

Silicon

2316.51

Si-HStreching

Silicon

1745.58

C=O Stretching

Cyclopentanone

1649.14

C=C Stretching

Conjucated Alkene

1658.18

C-C Stretching

Alkene

1244.09

C-N Stretching

Amine

1161.15

C-O Stretching

Tertiary alcohol

1002.98

C-F Stretching

Fluoro compounds

858.32

C-CI Stretching

Halo compounds

769.60

C-H bending

1,2-disubstituted

713.66

C=C bending

Alkene

574.79

C-Br Stretching

Halo compounds

522.71

C-I stretching

Halo compounds

DISCUSSION

In FT-IR spectra analysis, this sample Chitrarathaichooranam exhibits the peak value at 3778.55, 3535.52, 3010.88, 2926.01, 2854.65, 2357.01, 2316.51, 1745.58, 1649.14, 1658.18, 1244.09, 1161.15, 1002.98, 858.32, 769.60, 713.66, 574.79, 522.71 having O-H stretch, C-H stretch, Si-H stretch, C=O stretching, C=C stretching, C-N stretching, C-F stretching, C-Cl stretching, C-H stretching, C-Br stretching, C-I stretchings.

This indicates the presence of some organic functional groups such asAlcohol, Alkane, Silicon, Cyclopentanone, Conjucatedalkane,Alkene, Amine, Tertiary alcohol, Fluoro compound, Halo compound, 1,2-disubstitutes. The presence of alkanes protects against bacteria and fungal infections.likewise the presence of other these identified functional groups in the medicinal compound are also responsible for their therapeutic function.

CONCLUSION

The instrumental analysis FTIR study for Chitrarathaichooranam shows the presence of functional groups through the stretch and bends which responsible for its functional activity. These findings will help for further preclinical and clinical studies in Chitrarathaichooranam.

REFERENCES

1. Vaithiyarathinam K.S. Murugesamuthaliyar, Gunapadam-mooligaivaguppu, published by Directrate of Indian medicine and Homeopathy chennai-106, year 1936.

2. Nadkarni K.M. Indian Material Medica and their therapeutics – Vol. I, published by Ramdassbhatkal for popular prakashanpvt .Ltd, Mumbai.

3. Dr. S. Somasundaram, M.Sc., M.Phil., E.S.M.P., Ph.D., MaruthuvaThavaraIyal, vol – I, 1997 edition, ElangovanPathipagam, Tirunelveli – 2.

4. Dr. Anaivaari R. Anandan, Ph.D., Dr. M. Thulasimani, M.D.(Pharm)., Siddha MateriaMedica (Mineral and Animal kingdom), 2008 edition, Dept. of Indian medicine and Homeopathy, Chennai – 106.