FTIR CHARACTERIZATION OF SIDDHA MEDICINE NANNARI CHOORANAM

G.Ragavi1, R.Antony Duraichi2

1.P.G Scholar, Department of Gunapadam, Govt. Siddha Medical College, Palayamkottai.

2.Lecturer Grade II, Department of Gunapadam, Govt. Siddha Medical College, Palayamkottai.

Abstract

Background: The Nannarichooranam is a herbal medicine used for treating Diabetes, Leucorrhoea, Thirst, Burning micturition, and used as tonic, diuretic, health promoter also[1].

Objectives: To characterize the herbal drug “Nannarichooranam

Materials and Methods : The ingredient used in this preparation is Nannari root. The drugs was prepared as per siddha literatureGunapadamMooligaivakuppu. The drugs was analyzed by using FTIR Spectrum.

Result: Characterization plays a major role to identify the nature of the drug for standardization. FTIR characterization shows the presence of some functional group such as Carboxylic acids, amine salts, silicon, Esters, alkene, nitro compounds, alkyl aryl ether, tertiary alcohol, Fluoro compounds, Halo compounds, 1,2-disubstituted where identified in Siddha herbal formulation“Nannarichooranam”. This study forms the base for the pharmaceutical analysis ofNannarichooranam which will be followed by safety and efficacy studies later.

Conclusion: The instrumental analysis FTIR study for Nannarichooranam shows the presence of functional groups through the stretch and bends which responsible for its functional activity. It was to subject for further many studies to validate its efficacy and safety through proper standardization procedure. For its potency.

Keywords :FTIR, Siddha drug, Herbal formulation, Functional group

INTRODUCTION

The siddha system of medicine is mainly practised in the southern part of India as it originates from Tamilnadu, It is one of the earliest traditional medicine system in the world which treat not only the body but also the mind and the soul. According to siddha the five basic elements ( panchapootham) are present in all living and non-living things of the universe in various proportions. In the siddha system of medicine, all vital processes (physiological, biochemical-metabolic) have been classified under three functional heads namely vali, azhal, Iyyam (Vaatham, Pitham, kabam) are called three uyirthaathukal. Siddha medicine was formulated to treat various disease are some modern analytical equipments are helpful to get knowledge regarding the traditional medicinal compound formulation.

Nannarichooranam is indicated as one of the best siddha medicine for its wide medicinal uses. In ancient days it is widely used as a drug of choice fortreating Diabetes, Leucorrhoea, Thirst, Burning micturition, and used as tonic, diuretic, health promoter also[1].

In this study, FTIR characterization was done for the herbal Siddha formulation “Nannarichooranam ” to evaluate functional group. It is an excellent tool for quantitative analysis.

MATERIALS AND METHODS

Collection of raw materials

Nannari root was collected from surrounding places near Nagercoil.

Nannarichooranam is a siddha herbal formulation has the ingredients of

S.No.

Siddha name

Scientific name

Quantity

1.

Nannari

Hemidesmusindicus[2] [3],

S.Q

.

Process of preparation

Purified the Nannari root by removing the outer skin and dried it completely. Then it is placed in iron mortorand powdered well [1],[4].Then it was sieved through the thin clean white cloth. Finallychooranam was obtained and kept in an airtight container.

Dose

1 – 2gm

Adjuvant

Milk or hot water or sugar candy[1]

Indication

Diabetes, Leucorrhoea, Thirst, Burning micturition, and used as tonic, diuretic, health promoter also[1].

Details regarding the analysis

FTIR spectra were recorded at kalasalingam academy of research and education (International research center) Srivilliputhur.

FTIR Spectrum analysis

Fourier transform infrared spectroscopy it is an important and more advanced technique. It is used to identify the functional group to determine the quality and consistency of the sample material and can determine the amount of compound present in the sample.

In FTIR- infrared is passed from a source through a sample. This infrared is absorbed by the sample according to the chemical properties and some are transmitted. The spectrum that appears denotes the molecular absorption and transmission. It forms the molecular finger print of the sample. It is recorded as wavelength and the peaks seen in the spectrum indicate the amount of material present.

RESULTS

Fig.1. Image of the FTIR spectrum.

Table.1: FTIR Data interpretation of SAMPLE (AOP)

Wave number

Vibrational modes of SAMPLE (AOP) In IR region

Functional groups

3292.49

O-H Stretching

Carboxylic acid

2929.87

N-H Stretching

Amine salts

2856.58

N-H Stretching

Amine salts

2362.80

Si-H Stretch

Silicon

1743.65

C=O Stretching

Esters

1710.86

C=O Stretching

Carboxylic acid

1664.57

C=C Stretching

Alkene

1521.54

N-O Stretching

Nitro compound

1246.02

C-O Stretching

Alkyl aryl ether

1159.22

C-O Stretching

Tertiary alcohol

1001.06

C-F Stretching

Fluoro compounds

858.32

C-CI Stretching

Halo compounds

769.60

C-H bending

1,2-disubstituted

709.80

C=C bending

Alkene

576.72

C-Br Stretching

Halo compounds

520.78

C-I Stretching

Halo compounds

435.91

C-I Stretching

Halo compounds

DISCUSSION

In FT-IR spectra analysis, this sample Nannarichooranam exhibits the peak value at 3292.49,2929.87, 2856.58, 2362.80, 1743.65, 1710.86, 1664.57, 1521.54, 1246.02, 1159.22, 1001,06, 858.32, 769.60, 576.72, 520.78, 435.91 having O-H stretching, N-H stretching, Si-H stretching, C=O stretching, N-O stretching, C-O stretching, C-F stretching, C-CI stretching, C-H stretching, C-H bending, C=C bending, C-Br stretching, C-I stretching.

This indicates the presence of some organic functional groups such asCarboxylic acids, amine salts, silicon,Esters, alkene, nitro compounds, alkyl aryl ether, tertiary alcohol,Fluoro compounds, Halo compounds, 1,2-disubstituted.

The presence of alkanes protects against bacteria and fungal infections.likewise the presence of other these identified functional groups in the medicinal compound are also responsible for their therapeutic function.

CONCLUSION

Nowadays it is very essential to validate the traditional formulations to get various knowledge regarding the science behind those formulations. The FTIR characterization findings of Nannarichooranam helps to standardize this drug. Pharmacological and clinical trial have to be carried out on this formulation. These results will help in structural identification of this drug and further research on this drug.

REFERENCES

1. Vaithiyarathinam K.S. Murugesamuthaliyar, Gunapadam-mooligaivaguppu, published by Directrate of Indian medicine and Homeopathy chennai-106, year 1936.

2. Nadkarni K.M. Indian Material Medica and their therapeutics – Vol. I, published by Ramdassbhatkal for popular prakashanpvt .Ltd, Mumbai.

3. Dr. S. Somasundaram, M.Sc., M.Phil., E.S.M.P., Ph.D., MaruthuvaThavaraIyal, vol – I, 1997 edition, ElangovanPathipagam, Tirunelveli – 2.

4. Dr. Anaivaari R. Anandan, Ph.D., Dr. M. Thulasimani, M.D.(Pharm)., Siddha MateriaMedica (Mineral and Animal kingdom), 2008 edition, Dept. of Indian medicine and Homeopathy, Chennai – 106.